|
|
Riding hall
Poláček, Petr ; Šmak, Milan (referee) ; Sýkora, Karel (advisor)
Bachelor thesis with the disign of the steel load-bearing structure of riding hall. Trackway area has plan dimension of 25 x 60 m. Span of main frame is 36 m. Main frame are distanced 9 m. The hall is 10,5 m hight. The structure is design for the area of Svitávka near Letovice.
|
|
|
Diverzita endoparazitů lišek polárních
PAVLOVIČOVÁ, Markéta
The foxes represent a reservoir of zoonotic infections therefore it is important to be familiar with an occurrence of possible diseases. Arctic fox (Vulpes lagopus) faeces were collected between 2003 and 2011 from various locations on Bylot Island, Canada. These samples were consequently treated with microscopic and molecular methods to prove the presence of microsporidia, cryptosporidia and giardia. The presence of a specific DNA of Cryptosporidium was detected in two samples and specific DNA of Microsporidia was detected in 34 samples for genus Encephalitozoon and in five samples for genus Enterocytozoon. None of the animals showed any symptoms of a diarrhoea. Subsequent analyses showed the presence of the Cryptosporidium canis fox genotype, Encephalitozoon cuniculi II and III genotypes and Encephalitozoon hellem genotype 1A. None of the examined faeces samples were positive for the presence of specific G. intestinalis DNA. Results of the thesis show that Artic foxes are mainly infected by microsporidia of the genus Encephalitozoon, while the occurrence of other observed parasites was rare.
|
|
|
Noninvasive measurement of steroid homones and effect of hormonal manipulation on behaviour in the gecko Paroedura picta
Matušková, Lucie ; Kratochvíl, Lukáš (advisor) ; Fraňková, Marcela (referee)
Hormones influence life of all animals. Not only they affect physiological changes in organisms, but also impact their behaviour. This work focuses at two main groups of steroid hormones: glucocorticoids and androgens. Glucocortiods are activated in response to stress. Their levels can be measured using non-invasive methods, which have a range of advantages. The main advantage is the feedback-free sample collection for enzyme immunoassay. As the measurement involves metabolites of the hormones rather than the hormones themselves, prior validation of the method is, however, necessary. This work reports on a study aiming to validate non-invasive measurement on the Madagascar Ground Gecko (Paroedura Picta). The validation was based on ACTH challenge test: Synacthen Depot was injected, which should lead to increased blood level of glucocorticoids. The validation, however, was not successful. The measurement did not discover significant increase in the levels of the metabolites of glucocorticoids. In addition, the work focuses on behavioural effects of testosterone, the primary androgen. Hormonal manipulations have been carried out on several male and female specimens. The results have discovered differences in sexual behaviour between control groups. On the other hand, the hormonal manipulations had no...
|
|
|
Ecological aspects affecting microbiota in the digestive tracts of birds
Schmiedová, Lucie ; Kreisinger, Jakub (advisor) ; Mrázek, Jakub (referee) ; Gvoždíková Javůrková, Veronika (referee)
The results indicate that songbird GM is dominated by the bacterial phyla Proteobacteria, Firmicutes, Actinobacteria, Tenericutes, Bacteroidetes and Chlamydia, a community that differs from that of mammals. A t the interspecies l evel, GM composition is mainly influenced by phylogeny and less so by sampling site. While ecological factors , and especially diet , are important GM predictors in mammals, the effect of ecological factors , including diet , have a much smaller effect on inte r - and intraspecific variability in songbird GM composition . Our data from barn swallows ( Hirundo rustica ) suggest an effect of social GM transmission among adults forming social pair s, as well as trans - generational transmission from a social mother to her own nestlings. At the same time, we observed differ ing GM in adults and nestlings, with no dramatic change in GM with nestling age, a process previously recognised in mammals. Part of the GM detected appears to remain stable over time , and is therefore su itable for further detailed study. To conclude, our studies demonstrate that s ongbird GM differs from that of mammals and that the factors influencing GM composition appear to differ from those in mammals , thereby highlighting the importance of study ing mu ltiple vertebrate groups (not just mammals ) when...
|
|
|
Prostate Cancer Diagnostic Algorithm
Sedláčková, Hana ; Hora, Milan (advisor) ; Baxa, Jan (referee) ; Záleský, Miroslav (referee)
Prostate cancer diagnostic algorithm Aim: The aim of the study is to implement the latest scientific knowledge in the diagnosis of prostate cancer (PC). We focused on tumor markers, imaging methods, prostate biopsy methodology and we created a diagnostic algorithm based on a review of current literature in combination with our own experience. Material and methods: The algorithm is divided into several branches, which have been individually subjected to clinical studies. Due to the low sensitivity and specificity of PSA, prostate health index (PHI) was added to the first line of patient stratification. 787 patients were primarily examined and these subsequently underwent radical prostatectomy. PHI levels were compared with definitive staging and grading. Cut-off values for PC detection and high-risk stratification, including locally advanced PC were determined. Next, 320 patients underwent prostate biopsy followed by radical prostatectomy. The cohort was further divided into two subgroups, patients with GS = 6 and patients with GS > 6. The ability of PHI to distinguish between insignificant and significant prostate cancer was evaluated. In a multicentric study with 395 patients, PHI with additional markers (tPSA, PSAD) and multiparametric magnetic resonance imaging of prostate (mpMRI) was assessed....
|
|
|
Problematika endoparazitóz u koní v chovech na Brněnsku
Dvořáková, Pavlína
The aim of this diploma thesis was to evaluate the issue of endoparasitosis in horses in breeding in Brno and its surroundings. From November 2015 to January 2017, four stables with different numbers of horses with their different dispositions (age, sex, horse performance) were monitored. In total, 30 horses were observed and 450 samples of equine faeces were collected and analyzed. Samples were analyzed using flotation methods using the Breza solution. It was found that 82 % of the samples were infected with 0-5 eggs of Strongylidae parasites. The work has shown that the number of parasites egg negatively affect the horse health. The resistance of parasites to deworming products was not proven.
|
|
|
Zhodnocení prostorového chování a početnosti prasete divokého v lesním prostředí na základě analýzy distribuce trusu
Hrubý, Petr
The wild boar (Sus scrofa) is highly adaptable. This proves that it benefits in today's cultural and intensely exploited landscape. In the summer, it resides in fields where there is enough food, calm and causes damage to crop. During the winter period it moves to forests where conditions are less favourable. It is extensively hunted, disturbed by forest cuting, tourism and other human activities. In the winter time, it has only limited food sources in the woods, and therefore searches and visits the feeding sites. The escalating problems regarding the wild boar were an initiative to study his behavior. The behaviour was studied by analyzing the distribution of faecal pellet groups in the Bradlo forest complex with an area of 11.59 km2. The complex is situated in the northeast of the Czech Republic and is surrounded by agricultural landscapes. The distribution of faecal pellet groups was evaluated on 617 research areas (each covering an area of 100 m2), which were evenly distributed throughout the forest complex. In the first year (2017) was counted 20.4 individuals per km2. In the second year (2018) after intensive hunting during the year, 15.1 individuals were counted per km2. The research has examined the relationship between the amount of dropping and the factors such as geomorphological characteristics, distance from the feeding sites, water sources, forest edges, roads, hiking trails and intravilans. Impact of forest environment and Jurisdiction to the hunting area were investigated. It has been confirmed that most wild boar are staying in young dense forest stands, regardless of jurisdiction to hunting area, distance from roads, hiking trails, intravilans and areas with intensive forest cuting. With increasing distance from the edge of the forest, the number of droppings increased, and, conversely, with increasing distance from the feeding sites the number of droppings was smaller. The influence of geomorphological properties and distance from water courses has not been proved.
|
|
|
Studium prevalence \kur{Giardia intestinalis}
BROŽOVÁ, Kristýna
The present project is focused on the study of a prevalence of the gut protist, Giardia intestinalis, in human and animal samples using two diagnostic approaches: (i) coproscopical diagnostics, specifically Sheather flotation technique, and (ii) PCR. First, three PCR protocols for different genes (triosephosphate isomerase, beta-giardin and small ribosomal subunit) were optimized by gradient PCR. Based on these result, the gene TPI was chosen as the best option for molecular diagnostics of G. intestinalis in the collected stool and fecal samples. Giardia was detected only in the animal samples (2/41, dog and rabbit samples), all the analysed human samples remained negative. Further, the TPI protocol showed low sensitivity for diagnostics of G. intestinalis.
|
|
|
Zhodnocení faktorů ovlivňujících intenzitu poškození porostů na území podniku Kolowratovy lesy
Svoboda, Tomáš
The work assesses the factors affecting the intensity of damage to vegetation Europen deer and sika deer on the holding Kolowrat forests. The study is to compare the population density of each game species, found less used method of counting dung piles on the strips transect, where the resulting states do not correspond to states of documents supplied by the owner of the hunting grounds. The observed population density is further compared with the number of trees damaged by bark consumption. The result of this comparison is the finding that the damages do not increase proportionally with the number of animals. The work concludes with a proposal of measures to reduce damage, arising out of tree species in the surveyed area.
|
|
|
Riding hall
Poláček, Petr ; Šmak, Milan (referee) ; Sýkora, Karel (advisor)
Bachelor thesis with the disign of the steel load-bearing structure of riding hall. Trackway area has plan dimension of 25 x 60 m. Span of main frame is 36 m. Main frame are distanced 9 m. The hall is 10,5 m hight. The structure is design for the area of Svitávka near Letovice.
|
guest ::
